Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METLQHLILHDMPNSEEIEAVKSG----DHTLTYKGYRKRINQLANAMLQKGIQKGDRVALLCKNGHPASTVMFAALEIGAVVVPVSWQLKPYEMTGILKASEPKAMFYGAEFKEILDEVLPE---LSSLCVTMETGTAYETSAEFEALFAGPD-------HLPETEMVSPDDTALLMFTSGTTGNPKRCMITHGGIYRYVKKSNS---SIARMKGLRFLACHPIYHTSALICIMLGTFAETTFVFTKDQDPVHMLKVIEEEKIQTVMALPVFYTYLLEAWEKHQTDLSSLVILMTGGTKVPSSLISRYLD-IG-IPLAHGYGSTEAWGISTWTPDMGMDKAASAGKPVAGVKVKVEDPLTGEELPQGEIGEIVVHTPFLFKGYEDNPEATAKVLQ-NGWFRTGDSGYVDEDGFIFITGRYKDVIIYGGDNVYPDQVEEVIQQIPGILETAVVGIPDPLYGEKPKAFIVKNGGQRITEEDVIAFCKERLSAYK-IPE-VEFVNELPKNNLGKVKKDVLRNQAVHS 2D1S Chain:A ((24-543)) --SAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKSCCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVI-LDSKVDYRGYQCLDTFIKRNTPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENIVTRFSHARDPIYGNQVSPGTAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTSVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAIIITPEG-DDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKIDGRAIREILKK-

General information: TITO was launched using: RESULT: Template: 2D1S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3077 -286438 -93.09 -575.18 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -93.09 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_2D1S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2D1S-query.scw PDB file : Tito_Scwrl_2D1S.pdb: