Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDFAFKLYEYNVWANQQIFNRLKELPKEIYHQEIQSVFPSISHVLSHVYLSDLGWIEVFSGKT----LSDALALA--EQLKEQTEAKEIEEMEDLFLRLSERYILFLQQ--KEQLNKPLQIQNPSSGIMKTTVSELLPHVVNHGTYHRGNITAMLRQAGYASAPTDYGLYLFMTKTEKA 2NSF Chain:A ((31-178)) ------ERLTLARLGTSHYSRQLSLVDNAEFGEHSLLEGWTRSHLIAHVAYNAIALCNLMHWANTGEETPMYVSPEARNEEIAYGSTLNPDALRNLHEHSVARLDVAWRETSEDAWSHEVLTA--QG--RTVPASETLWMRSREVWIHAVDLGAVATF----------------------

General information: TITO was launched using: RESULT: Template: 2NSF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 578 -51362 -88.86 -366.87 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -88.86 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.388

(partial model without unconserved sides chains): PDB file : Tito_2NSF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NSF-query.scw PDB file : Tito_Scwrl_2NSF.pdb: