Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKKVILAAFILVGSTLGAFSFSSDASAKHVNGNITWYNGVGKKGSSGKKLGH-WDC--AT-KIGFDV-----PRNGTKIRAYAKAKPKKVITV-YKNDVGRMPNAVLDVSPKAFKALGYPLSKGKVAGHYSY 3D30 Chain:A ((4-105)) ---------------------------DDLHEGYATYTGSGYSGGAFLLDPIPSDMEITAINPADLNYGGVKAALAGSYLEVEG-PKGKTTVYVTDLYPEGARGA--LDLSPNAFRKIGN-MKDGKINIKWRV

General information: TITO was launched using: RESULT: Template: 3D30.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 454 -31541 -69.47 -342.83 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -69.47 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_3D30.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D30-query.scw PDB file : Tito_Scwrl_3D30.pdb: