Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIEIIISLGIYFIAMLLIGWYAFKKTTDINDYMLGGRGLGPFVTALSAGAADMSGWMLMGVPGAMFATGLSTLWLALGLTIGAYSNYLLLAPRLRAYTEAADDAITIPDFFDKRFQHSSSLLKIVSALIIMIFFTLYTSSGMVSGGRLFESAFGADYKLGLFLTT----AVVVLYTLFGGFLAVSLTDFVQGAIMFAALVLVPIVAFTHVGGVAPTFHEIDAVNPHLLDIFKGASVISIISYLAWGLGYYGQPHIIVRFMAIKDIKDLKPARRIGMSWMIITVLGSVLTGLIGVAYAHKFGVAVKDPEMIFIIFSKILFHPLITGFLL--SAILAAIMSSISSQLLVTASAVTEDLYRSFFRRKASDKELVMIGRLSVLVIAVIAVLLSLNPNSTILDLVGYAWAGFGSAFGPAILLSLYWKRMNEWGALAAMIVGAATVLIWITTGLAKSTGVYEIIPGFILSMIAGIIVSMITKRPAKASYRLFGVMEKLLKRKK 1JKM Chain:A ((13-370)) ------------------------------------------------------PGRLGDESSGPRTDPRFSPAMVEALATFG------LDAVAAAPPVSASDDLPTVLAAVGASHDGFQAVYDSIALDLPTDRDDVETSTETILGVD------GNEITLHVFRPAGVEGVLPGLVYTHGGGMTILTTDNRVHRRWCTDLAAAGSVVVMVDFRNAWTAEGHHPFPSGVEDCLAAVLWVDE------------------------------HRESLGLSGVVVQGES------GGGNLAIATTLLAKRRGRLDAIDGVYASIPYISGGYAWDHERRLTELPSLVENDGYFIENGGMALLVRAYDPTGEHAE---------------------DPIAWPYFASEDELRGLP----PFVVAVNELDPLRDEGIAFARRLARAGVDVAARVNIGLVHGADVIFRHW-------------LPAALESTVRDVAGFAADRARLR

General information: TITO was launched using: RESULT: Template: 1JKM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2154 -277960 -129.04 -789.66 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -129.04 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.260

(partial model without unconserved sides chains): PDB file : Tito_1JKM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JKM-query.scw PDB file : Tito_Scwrl_1JKM.pdb: