Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRYLTAGESHGPQLTTIIEGVPAGLYITEEDINFELARRQKGHGRGRRMQIEKDQAKIMSGVRHARTLGSPIALVVENNDW-KHWTKIMGAAPITEDEEKEMKRQISRPRPGHADLNGAIKYNHRDMRN-VLERSSARETTVRVAAGAVAKKILSE-LGIKVAGHVLQIGAVKAEKTEYTSIEDLQRVTEESPVRCYDEEAGKKMMAAIDEAKANGDSIGGIVEVIVEGMPVGVGSYVHY-DRKLDSKLAAAVLSINAFKGVEFGIGFEAAGRNGSEVHDEIIWDEEKGYTRATNRLGGLEGGMTTGMPIVVRGVMKPIPTLYKPLKSVDIETKEPFSASIERSDSCAVPAASVVAEAAVAWEIANAVVEQFGLDQIDRIRENVENMRKLSREF 4OB9 Chain:A ((10-354)) FRVTTCGESHGVGLMAIVDGVPPGLALTEEDLQKDLDRRKPGTSKFATQRKEPDQVEIISGVFEGKTTGTPIGLLIRNTDQK----------------------------------------------GGG--RSSARETAMRVAAGAIAKKYLAEKFGVLIRGHVTQIGNEVAE-KL------DWNEVPNNPFFCGDVDAVPRFEALVTSLREQGTSCGAKLEILAEKVPVGWG--EPVFD-RLDADIAHAMMSINAVKGVEIGDGFAVAGQFGHETRDELTSH-----GFLANHAGGILGGISSGQTIRVAIALKPTA--------------------KGRHDPCVGVRATPIAEAMLAIVLMDHFLRHRAQNADV----------------

General information: TITO was launched using: RESULT: Template: 4OB9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1504 -32349 -21.51 -111.16 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -21.51 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_4OB9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OB9-query.scw PDB file : Tito_Scwrl_4OB9.pdb: