Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------MNNRII------------YVVSDSVG--ETAELVVKAALSQFNGSADDTHVRRI--PYVEDIGTINEVISLAKAD-GGIICFTLVVPEIREYLIAEAEKANVLYYDII----------------GPLIDKMETAYGLTAKYEP-----GRVRQLDEDYFKKVEAIEFAVK-----YDDGRDPRGILKADIVLIGVSRTSKTPLSQYLAH---KRLKVANVP---IVPEVDP----PEELFNVDPKKC--------------------------IGLKISPDKLNHIRKERLKSLGLND----KAIYANINRIKEELEYFEKIVDRIGCQVVDVSNKAVEETANIIHHLKTKNI----------------- 2V4J Chain:B ((2-381)) AFISSGYNPEKPMANRITDIGPRKFDEFFPPVIAKNFGSWLYHEILEPGVLMHVAESGDKVYTVRVGAARLMSITHIREMCDIADKYCGGHLRFT--TRNNVEFMVADEASLKALKEDLASRKFDGGSLKFPIGGTGAGVSNIVHTQGWVHCHTPATDASGPVKAIMDEVFEDFQSMRLPAPVRISLACCINMCGAVHCSDIGVVGIHRKPPMIDHEWTDQLCEIPLAVASCPTAAVRPTKLEIGDKKVNTIAIKNERCMYCGNCYTMCPALPISDGEGDGVVIMVGGKVSNRISMPKFSKVVVAYIPNEPPRWPSLTKTIKHIIEVYSANAYKYERLGEWAERIGWERFFSLTGLEFSHHLIDDFRDPAYYTWRQSTQFKF

General information: TITO was launched using: RESULT: Template: 2V4J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1130 3871 3.43 14.44 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : 3.43 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.221

(partial model without unconserved sides chains): PDB file : Tito_2V4J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V4J-query.scw PDB file : Tito_Scwrl_2V4J.pdb: