Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRVFVARQPIFNRKEQVVAYELLYRESEENVYSAKDGDQATTDLVINSFLNIGIEKLTEGKRCFVNFTESLMFSNLPTSFNPKQLVIEILEDIPITPALISRCKELKKMGYMLALDDFYAINPQDEDLLEKLMSYIDILKIDFLKTTRMERRTILQTYGCRGLIFLAEKVETRKEYKQAVQDGFQLFQGYFFSEPRIISGHDLSTHFYSYYELLNELSKEQPNIKRVTEYIERDLSLSYQILKFLNSSHSRLSQKIESIQQAIMLLGFNEIKRWIYILSFKDL-SRKG-HSSKHEIIKISLIRAKLCELLARKTSRPQPASYMLIGMFSLIDTLLHREI-----------------------------------EEIVQELPLKDEVGQALLGHQND---------YYQMLELVKLIESNNWDTCSELGNQLDKEEAYECYLEALEWCHNLMDAK 3HC1 Chain:A ((23-242)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VDELPTVPDIVLRIAGKLNDPDVAIDEVADLLLQDQVLTARVVHLANSPLYSAARPISSIRDAVIYLGLDLLREAIFTCAIVDLFKTGKGPLNRSTLWAHSLGVARIAKLIAERTGFLNPVNVYVAGLLHDVGEVFINFFRGKEFSQVVTLVDEEKITFGQAEERLFGTSHCEVGFALAKRWSLNEFICDTILYHHDIEAVPYKQAAIVAMVAFADEYCT------------------------------------

General information: TITO was launched using: RESULT: Template: 3HC1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 692 -87700 -126.73 -504.02 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -126.73 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_3HC1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HC1-query.scw PDB file : Tito_Scwrl_3HC1.pdb: