Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVQRKKISLIGAGNIGGALAHMVTLRELGDVVLFDVNDGIPQGKALDIAESSPIGGFSVNIIGTNRYGDIKNSDAIIITAGIARKPG-----MSRDDLLQTNAKVMKEVGENIRKYSPNAFVIVVTNPLDAMVSVVHKFSNLPANMIVGMAGVLDSSRFRYFLARELNISVEDVSAFVLGGHGDTMVPLIRCASIAGIPLTQIIDMGLITQEKVDEIVKRTRNGGKEIIDLLKSGSAYYAPASSSIYMLESYLRDEKRILPCATYLNGEYGVKDLFIGVPVIIGKNGIEKVLEVKMDDSEQEMFNKSVNAVKELVKSLSL 4PLY Chain:E ((3-318)) ---QRKKISLIGAGNIGGTLAHLIAQKELGDVVLFDIVEGMPQGKALDISHSSPIMGSNVKITGTNNYEDIKGSDVVIITAGIPRKPGKSDKEWSRDDLLSVNAKIMKDVAENIKKYCPNAFVIVVTNPLDVMVYVLHKYSGLPHNKVCGMAGVLDSSRFRYFLAEKLNVSPNDVQAMVIGGHGDTMVPLTRYCTVGGIPLTEFIKQGWITQEEIDEIVERTRNAGGEIVNLLKTGSAYFAPAASAIEMAESYLKDKKRILPCSAYLEGQYGVKDLFVGVPVIIGKNGVEKIIELELTEEEQEMFDKSVESVRELVETVKK

General information: TITO was launched using: RESULT: Template: 4PLY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -304645 for 2808 contacts (-108.5/contact) + 2D Compatibility (PS) -33335 + (NN) -14237 + (LL) 452 1D Compatibility (HY) -40400 + (ID) 10800 Total energy: -402965.0 ( -143.51 by residue) QMean score : 0.621

(partial model without unconserved sides chains): PDB file : Tito_4PLY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PLY-query.scw PDB file : Tito_Scwrl_4PLY.pdb: