Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKQIISWITDYQNTGDEAVLRQVREVCWPIVEAVLQEKVMD-DEQANNLREKGIERFPFI--------ISKYQA------DVQLPVETFLQNTYRFYFHQVMRESS 2FI0 Chain:A ((13-68)) ---------------------------------IPVAEVVDKHPEVLEILVELG---FKPLANPLMRNTVGRKVSLKQGSKLAGTPMDKIVR---------------

General information: TITO was launched using: RESULT: Template: 2FI0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 104 -17547 -168.72 -427.96 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -168.72 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.599

(partial model without unconserved sides chains): PDB file : Tito_2FI0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FI0-query.scw PDB file : Tito_Scwrl_2FI0.pdb: