Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAMNLRKNQAPLYIKVHEIDNTAIIVNDGGLPKGTVFSCGLVLEEDVPQGHKVALTDL--NQGDEIVRYGEVIGFADETIKRGSWIREA--LVRMPAPPALDDLPLANRVPQPRPPLEGYTFEGYRNADGSAGTKNILGITTSVQCVVGVLDYAVKRIKEELLPKYPNVDDVVPLHHQYGCGVAINAPDAVIPIRTIQNLAKHPNFGGEVMV-----IGLGCEKLLPERIASEND--DDILSLQDHRGFAAMIQSILEMAEERLIRLNSRTRVSCPVSDLVIGLQCGGSDAFSGVTANPAVGYAAD-----LLVRAGATVLFSEVTEVR------DAIHLLTPRAVSEEVG-------QSLIKEMKWYDSYLRRGDADRSANPSPGNKKGGLSNVVEKALGSVAKSGTSP--ISGVLGPGE------RAKQKGLLFAATPASDFVCGTLQLAAGMNLQVFTTGRGTP----YGLAAAPVLKVSTRHSLSEHWADLIDINAGRIATGEASIED--VGWEIFRTILDVASGRKQTWADRWGLHNDLCLFNPAPVT 3EPO Chain:A ((5-546)) ----STIKAVAETISTGPIPGSRKVYQAGELFPELRVPFREVAVHPSANEPPVTIYDPSGPYSDPAIQIDIEKGLPR---TREALVVARGDVEEVADPRQVPEFPDTGRKIYRAKPGKLVTQLEYARAGI---------ITAEMEYVAIRENLRREQDRPCVRDGEDFGASIPDFVTPEFVRQEIARGRAIIPANINHGELEPMAIGRNFLVKINANIGNTVADEVDKLVWATRWGADTVMDLSTGRN--------IHNIRDWIIR-NSSVPIGTVPIYQALEKVNGVAEDLNWEVFRDTLIEQCEQGVDYFTIHAGVRLPFIPMTAKRVTGIVSRGGSIMAKWCLAHHKENFLYERFDEICEIMRAYDVSFSLGDGLRPGSTADAN--DEAQFSELRTLGELTKVAWKHGVQVMIEGPGHVAMHKIKANMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKTGVITYKLAAHAADLAKGHPGAAMWDDAISRARFEFR-WEDQFNLGLDPETARKFH--

General information: TITO was launched using: RESULT: Template: 3EPO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2624 -81907 -31.21 -170.64 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -31.21 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.231

(partial model without unconserved sides chains): PDB file : Tito_3EPO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EPO-query.scw PDB file : Tito_Scwrl_3EPO.pdb: