Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITLFQCLYLILFSFICYQGAAAFSHSTAASWLAAALGAAAAGLYIWNTKRVWKHCSSGLCAWIAVIQVMSVGVVLIGTDIMPVLCVIAIFAGCEGLRIGQSALQARLSDQIDKLTQAEQHANQMLIDVRSRNHDTMKHITAIKSA--------QP-----KADTQAYIQNWADQYSQYDRFLKG-----------------ENAYVAGVLYDFLEKARASNV---SVSL-----HMHTPLSSLPFSPADQVSLVGNILENALDSAAEARE----KAEIKLETSLRSGLYVLTCENSTPGMDPKVLDTIYQSFGRSTKN-----------GAHEGMGTYIIQKLVKGAFGRLDFTYRHP-IFRLEIKIPFQK 2Q8G Chain:A ((105-366)) ---------------------------------------------------------------------------------------------------------------------------AIYDFTDTVIRIRNRHNDVIPTMAQGVIEYKESFDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGKHIGSINPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHANRGVYPPIQVHVTLGNEDLTVKMSDRGGGVPLRKIDRLFN-YMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKAL-

General information: TITO was launched using: RESULT: Template: 2Q8G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 796 -93092 -116.95 -482.34 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -116.95 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.452

(partial model without unconserved sides chains): PDB file : Tito_2Q8G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q8G-query.scw PDB file : Tito_Scwrl_2Q8G.pdb: