Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MVNKQLKKKAQEIGNVPTHYELEIEDYDQKQK-KYGQAYFIWKDPKDPEKHITVELRNDG--------ALLTFSTTVHSETDKKL------------PDAELKLTALQFAAANHPG-----TFMNFHFQGKEERGQHIRFVYTKMELGLPIPNSGFLIDMTRSGQIVHFLYYGEGHKAEVPTEFVAKEKVVSHYLNTMSF--ELMYDVI--DGEQEPRLVYEPILPGYSYPADVDEIVPDQHIADERIENTAPLPPLQNKEEVDIFALLGFTSDMQKVSERDFGEQIGSTWRRGAAPERKDLSIGSYFETRNKNTIKMKTDKRTGKLKAALSFMDWRNNLQCST---EECQEIALQFLYALYPRAAEFFRVNPVRIDERGRVRNHFSVWYKGVPLRFGAARIIVNPETGLIDAFMAPDIEPEQLEAINHRPDVSAEEAKEAFLAAFDVKLEWQPDFTAGSDQH-CKLVYKPVYPSYIDAHIRKKKRL 4R33 Chain:A ((35-418)) GDFVLPELEDVRAEAATVDTRAVLALAEGEEPAESRAAVALALWEDRSIGTAELQAAAEARCGARRPRLHTFVPLYTTNYCDSECKMCSMRKGNHRLDRKFSGRKEITEQLEILYHHEGVRGVGFLTGEYEDKHTRLASAFRIGWAIRTALDL---GF-------ERVYFNIGSMEQDEIDVLGEWIGREDPVTMCVFQESYDRETYRRFMGKTSVGVPKADFDRRVVSFDRWLD----------AGYRYVNPGVLVGLHDDLSAELVSLVAHGDHLRS---------------RGA---TADLSVPRMRPAMKSRDTTRVGDDDYLRLMSVVAFTCPEQRLVLTTREPQEFQDVALGLAGVISPGS---------------------------------------------------PDVAP-------YRAGCEARNDEKS--SQFLVADLRRPRHILGRIEASGTPVDHFVNPAGEASR-------

General information: TITO was launched using: RESULT: Template: 4R33.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1824 -104750 -57.43 -301.01 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -57.43 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_4R33.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R33-query.scw PDB file : Tito_Scwrl_4R33.pdb: