Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQPLVGIIMGSTSDWETMKHACDILDELNVPYEKKVVSAHRTPDFMFEYAETARERGIKVIIAGAGGAAHLPGMTAAKTTLPVIGVPVQSKALNGMDSLLSIVQMPGGVPVATTSIGKAGAVNAGLLAAQILSAFDEDLARKLDERRENTKQTVLESSDQLV 2FWP Chain:B ((23-167)) --PVVGIIMGSQSDWETMRHADALLTELEIPHETLIVSANRTPDRLADYARTAAERGLNVIIAGAGGAAHLPGMCAAWTRLPVLGVPVESRALKGMDSLLSIVQMPGGVPVGTLAIGASGAKNAALLAASILALYNPALAARLETWR---------------

General information: TITO was launched using: RESULT: Template: 2FWP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 831 -120302 -144.77 -829.67 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : -144.77 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.627

(partial model without unconserved sides chains): PDB file : Tito_2FWP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FWP-query.scw PDB file : Tito_Scwrl_2FWP.pdb: