Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKDKLRYAILKEIFEGN---TPLSENDIG-------VTEDQFDDAVNFLKREGYIIGVHYSDD---RPHLYKLGPELTEKGENYLKENGTWSKAYKTIKEIKDWIK 2DQL Chain:A ((24-104)) ------ICYILYVLLQGESYGTEL-IQQLETEHPTYRLSDTVLYSAIKFLEDNRAITGYWKKLEGRGRPR---RMYQVSPEWQHQAEDLAR----------------

General information: TITO was launched using: RESULT: Template: 2DQL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 209 -35724 -170.93 -525.35 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -170.93 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_2DQL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DQL-query.scw PDB file : Tito_Scwrl_2DQL.pdb: