Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASIAVCPGSFDPVTYGHLDIIKRGAHIFEQVYVCVLNNSSKKPLFSVEERCELLREVTKDIPNITVETSQGLLIDYAKRKNAKAILRGLRAVSDFEYEMQGTSVNRVLDESIETFFMMTNNQYSFLSSSIVKEVARYNGSVSEFVPPEVELALQQKFRQG 1VLH Chain:F ((15-169)) ----AVYPGSFDPITLGHVDIIKRALSIFDELVVLVTENPRKKCMFTLEERKKLIEEVLSDLDGVKVDVHHGLLVDYLKKHGIKVLVRGLRAVTDYEYELQMALANKKLYSDLETVFLIASEKFSFISSSLVKEVALYGGDVTEWVPPEVARALNEKLK--

General information: TITO was launched using: RESULT: Template: 1VLH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 728 -118455 -162.71 -764.23 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain F : 0.87 3D Compatibility (PKB) : -162.71 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_1VLH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VLH-query.scw PDB file : Tito_Scwrl_1VLH.pdb: