Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISNAKIARINELAAKAKAGVITEEEKAEQQ---KLRQEYLKGFRSSMKNTLKSVKIIDPEGNDVTPEKLKR------EQRNNKLH 2RNM Chain:A ((217-295)) -------MKIDAIVGRNSAKDIRTEERARVQLGNVVTAAALHGGIRISDQTTNSVETVVGKGESRVLIGNEYGGKGFWDNHHHHHH

General information: TITO was launched using: RESULT: Template: 2RNM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 212 30622 144.44 437.46 target 2D structure prediction score : 0.14 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 144.44 2D Compatibility (Sec. Struct. Predict.) : 0.14 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.075

(partial model without unconserved sides chains): PDB file : Tito_2RNM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RNM-query.scw PDB file : Tito_Scwrl_2RNM.pdb: