Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------MIREHLLKEIEK-KRAELLQIVMANGMTSH-IT-IELSQELDHLLIQYQKQRLRAVAGDE----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 4LB8 Chain:A ((18-290)) EIEVINDGTMIKFKDVESYENALLKVSAMSTSEQVSFLNSLSFKSQMILMQEADGELDKICNQAADKAEFDVLYEKYKHKYGDVFMFNTIDATDLSPYSRLVYVANEYFVNMKGEFMIGDSLVVDKVYTDFKERQQQFTVSTRSSVSDLSSINEAYSRQKDRKVGLYLSVSSGIIHANFTSQKKGVFGWSRYSTTYHAKVNLRGFEFAQGELLGYGPVYVNKDGIPFAIDTKEMGGNVTKVFGRKLAQECTGTIEIWSRGVPYDQRGFATVRL

General information: TITO was launched using: RESULT: Template: 4LB8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 115 8208 71.37 143.99 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : 71.37 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.636

(partial model without unconserved sides chains): PDB file : Tito_4LB8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LB8-query.scw PDB file : Tito_Scwrl_4LB8.pdb: