Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLEEKTIAKEQIFSGKVIDLYVEDVELPNGKASKREIVKH-------PGAVAVLAVTDEG----KIIMVKQFRKPLERTIVEIPAGKLEKGEEPEYTALRELEEETGYTAKKLTKITAFYTSPGFADEIVHVFL----AEELSVLEEKRELDEDEFVEVMEVTLEDALKLVESREVYDAKTAYAIQYLQLKEALQAQK 2DSD Chain:A ((20-186)) --------ISEELISEGKWVKLEKTTYMDPTGKTRTWESVKRTTRKEQTADGVAVIPVLQRTLHYECIVLVKQFRPPMGGYCIEFPAGLIDDGETPEAAALRELEEETGYKGDIAECSPAVCMDPGLSNCTIHIVTVTINGDDAENARPKPKPGDGEFVEVISLPKNDLLQRLDA-------------------------

General information: TITO was launched using: RESULT: Template: 2DSD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 636 37507 58.97 246.76 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 58.97 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.403

(partial model without unconserved sides chains): PDB file : Tito_2DSD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DSD-query.scw PDB file : Tito_Scwrl_2DSD.pdb: