Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNMDFNLFMNDIVRQARQEITAAGYTELKTAEEVDEALTKKGTTLVMVNSVCGCAGGIARPAAYHSVHYDKRPDQLVTVFAGQDKEATARARDYFEGYPPSSPSFAILKDGKIMKMVERHEIEGHEPMAVVAKLQEAFEEYCEEV 3EMX Chain:A ((224-347)) ---SLSYVKEGLAVLED------GRLIYITPEEFRQLL-QGDAILAVYSKTCPHCHRDWPQLIQASKEVDVPIVMFIWGSLIGERELSAARLEMNKAGVEGTPTLVFYKEGRIVDKLVG----ATPWSLKVEKAREIY-------

General information: TITO was launched using: RESULT: Template: 3EMX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 596 -55072 -92.40 -444.13 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -92.40 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.314

(partial model without unconserved sides chains): PDB file : Tito_3EMX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EMX-query.scw PDB file : Tito_Scwrl_3EMX.pdb: