Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence KRLVVQRVTEASVTVDEEVVGQ------IGQGLMVLVGITHDDTEDDAAYLADKVVNLRIFDDSEGKMNLSLVDIGGEILSVSQFTLYGDTKKGRRPNYMNAAKPDKALGLYEKWNDLLREK--GIKVETGTFGAMMDVQLTNSGPVTLIMDSK 4NBI Chain:B ((2-157)) -RVVIQRVKGAILSVRK-----LEIISEIKNGLICFLGIHKNDTWEDALYIIRKCLNLRLWNNDNKTWDKNVKDLNYELLIVSQFTLFGNTKKGNKPDFHLAKEPNEALIFYNKIIDEFKKQYNDDKIKIGKFGNYMNIDVTNDGPVTIYIDT-

General information: TITO was launched using: RESULT: Template: 4NBI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 624 17766 28.47 127.81 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : 28.47 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_4NBI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NBI-query.scw PDB file : Tito_Scwrl_4NBI.pdb: