Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MKMTSRKLSDILKQRLQHENRSFLFDREKDTLRVEDQTTKKGITLDLPPIIAKWELKKDEAIDEI---------VYYVS-----EAMTAMEGKAQEMTGKETRIYPVIRSTSFPDKSSEDIPLIYDDHTAETRIYYALDLGKTYRLIDQRMLEKENWTKERIRETAA--FNLRSLP-------TVVKEDTVAGNYFYFFRANDGYDASRILNEAI----LNEYKQHAEGELAISVPH-QDVLILADIRNESGYDILGQMSMSFFAGGTVPITALSFLYNEGKLEPVFILAKSRPKKD 3V3T Chain:A ((1-306)) MKNKIVFAPIGQGGGNIVDTLLGICGDYNALFINTSKKDLDSLKHAKHTYHIPKERKKAVGYAQTYYKQIIAQIMEKFSSCDIVIFVATMAGGAGSGITPPILGLAKQMYPNKHFGFVGVLPKATEDIDEHMNAIACWNDIMRSTNEGKDISIYLLDNNKREKESDINKEFATLFNDFMNMSESHAEGVVDEDEISKLLTMKKSNVILEFDDKEDIQVALAKSLKESIFAEYTTNTCEFMGISTTRVVDVEAIKSIVGYPRRTFKGYNS------KKNIVVATGIEPQKTTVQMMNEIIEDKMKQR

General information: TITO was launched using: RESULT: Template: 3V3T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1358 68489 50.43 260.41 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 50.43 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.238

(partial model without unconserved sides chains): PDB file : Tito_3V3T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V3T-query.scw PDB file : Tito_Scwrl_3V3T.pdb: