Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHSKTLLLTAAAGLMLTCGAVSSQAKHKLSDPYHFTVNAAAETEPVDTAGDAADDPAIWLDPKTPQNSKLITTNKKSGLVVYSLDGKMLHSYNTGKLNNVDIRYDFPLNGKKVDIAAASNRSEGKNTIEIYAIDGKNGTLQSMTDPDHPIATAINEVYGFTLYHSQKTGKYYAMVTGKEGEFEQYELKADKNGYISGKKVRAFKMNSQTEGMAADDEYGRLYIAEEDE-AIWKFSAEPDGGSNGTVIDRADGRHLTRDIEGLTIYYAADGKGYLMASSQGNSSYAIYDRQGKNKYVADFRITDGPETDGTSDTDGIDVLGFGLGPEYPFGIFVAQDGENIDHGQKANQNFKIVPWERIADQIGFRPLANEQVDPRKLTDRSGK 3M0C Chain:C ((558-632)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QWPNGITLDLLSGRLYWVDSKLHSISSIDVNGGN--RKTILED---EKRLAHPFSLAVF-----EDKVFWTDIINEAIFSANRLT--------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3M0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 341 -5544 -16.26 -74.92 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain C : 0.58 3D Compatibility (PKB) : -16.26 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_3M0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3M0C-query.scw PDB file : Tito_Scwrl_3M0C.pdb: