Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGELANCPKCNALFLKTKLQTVCQACIKEEEKSFETVYKFLRKQENRQSTLSRITEETGVEEELILKFIRQKRIQITHL--------PNLAYPCERCGTSIRE----GKFCKACQSDIKDQMDHLNHEDALKIEKENSKKDTYYAYNTKNS 2GMG Chain:A ((14-96)) ----------------------------------EKIIELLLE---GDYSPSELARILDMRGKGSKKVILEDLKVISKIAKREGMVLLIKPAQCRKCGFVFKAEINIPSRCPKCKSEWIE-------------------------------

General information: TITO was launched using: RESULT: Template: 2GMG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 131 -11929 -91.06 -168.01 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -91.06 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.403

(partial model without unconserved sides chains): PDB file : Tito_2GMG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GMG-query.scw PDB file : Tito_Scwrl_2GMG.pdb: