Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIAVVTDSTAYIPKEMREQHQIHMIPLQVVFREETYREEIELDWKSFYEEVKKHNELPTTSQPPIGELVALYEELGKSYDAVISIHLSSGISGTFSSAAAADSMV----DNIDVYPFDSEISCLAQGFYALKAAELIKNGASSPEDIIKELEEMKKTVRAYFMVDDLAHLQRGGRLSSAQAFIGSLLKVKPILHFDNKVIVPFEKIRTRKKAISRIYELLDEDASKGLPMRAAVIHANREEEAAKIIEELSAKYPHVEFYNSYFGAVIGTHLGEGALGICWCFK 1PZX Chain:A ((3-285)) MPIEIITDSGADLPQSYIREHRIAFLPLVVHWNGQDYKDGITIEPKQVYDAMRQ-GHTVKTAQPSPLAMKELFLPYAKENRPCLYIAFSSKLSGTYQTAMAVRSELLDEYPEFRLTIIDSKCASLGQGLAVMKAVELAKQNTP-YNLLCETIESYCRHMEHIFTVDNLDYLARGGRIS----------NIKPLLHVEDGALIPLEKWRGRKKVLKRMVELMGERGDDLQKQTIGISHADDEETALELKQMIEETHGCTRFFLSDIGSAIGAHAGPGTIALFFLNK

General information: TITO was launched using: RESULT: Template: 1PZX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1528 -69555 -45.52 -258.57 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -45.52 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_1PZX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PZX-query.scw PDB file : Tito_Scwrl_1PZX.pdb: