Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLKVMTVFGTRPEAIKMAPLVLELKKYPEIDSYVTVTAQHRQMLDQVLDAFHIKPDFDLNIMKERQTLAEITSNALVRLDELFKDIKPDIVLVHGDTTTTFAGSLAAFYHQIAVGHVEAGLRTGNKYSPFPEELNRQMTGAIADLHFAPTGQAKDNLLKENKKADSIFVTGNTAIDALNTTVRDG----YS----HPVLDQVGEDKMILLTAHR--RENLGEPMENMFKAIRRIVGEFEDVQVVYPVHLNPVVREAAHKHFGD-SDRVHLIEPLEVIDFHNFAAKSHFILTDSGGVQEEAPSLGKPVLVLRDTTERPEGVEAGTLKLAGTDEENIYQLAKQLLTDPDEYKKMSQASNPYGDGEASRRIVEELLFHYGYRKEQPDSFTGK 3DZC Chain:A ((24-395)) -AMKKVLIVFGTRPEAIKMAPLVQQLCQDNRFVAKVCVTGQHREMLDQVLELFSITPDFDLNIMEPGQTLNGVTSKILLGMQQVLSSEQPDVVLVHGDTATTFAASLAAYYQQIPVGHVEAGLRTGNIYSPWPEEGNRKLTAALTQYHFAPTDTSRANLLQENYNAENIFVTGNTVIDALLAVREKIHTDMDLQATLESQFPMLDASKKLILVTGHRRES-FGGGFERICQALITTAEQHPECQILYPVHLNPNVREPVNKLL-KGVSNIVLIEPQQYLPFVYLMDRAHIILTDSGGIQEEAPSLGKPVLVMRETTERPEAVAAGTVKLVGTNQQQICDALSLLLTDPQAYQAMSQAH----NPYGDGKACQRIADILA------------

General information: TITO was launched using: RESULT: Template: 3DZC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1885 -75996 -40.32 -210.52 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -40.32 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_3DZC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DZC-query.scw PDB file : Tito_Scwrl_3DZC.pdb: