Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKKELFDF-TNIT--PKLFTELRVADKTVLQSFNFDEKNHQIYTTQVASGLGKDNTQSYRITRLSLEGLQLDSMLLKHGGHGTNIGIENRNGTIYIWSLYDKPNETDKSELVCFPYKAGATL-DENSKELQRFSNMPFDHRVTPALDMKNRQLAIRQYDTKNNNNKQWVTIF----NLDDAIANKNNPLYTINIPDELHYLQGFFLDDGYLYWYTGDTNSKSYPNLITVFDSDN--KIVLQKEITVGKDLSTRYENNFREPEGICMYTNPETGAKSLMVGITSGKEGNRISRIYAYHSYENFMNHVPMLRSPLLKTVGHQDTPPERFQPFIQTFILEYNAQNKK-WMVPTSGYLPSYTSNLVRNITINADGNLQVTLNERYISLLHQSIEGDFRLKQKDIRMGSWYFAGGEKSNVLEIGFMKGSTKIRPDDAAISNASRMSIFMIVADKIEV 4UUW Chain:A ((1-394)) MERAEILGVGTELLYGETLDTNTAEIARS-LKPYALKVER-TLRVADEVAPLAREVEEAFARARLVVLS----------GGLGPTPDDVTREAVALALGEPLELDEAVLGEIEAFFRARGRAMPEANRKQAMRIPSATWLKNPRG----TAPGWWVRK------GGKDLVLLPGPPPEWRPMWQEVLPRLGLPRRPYAERVLKTWGIGESEIVERLGPLFVREEEVEVGTYPKVHGVEVVVRGREDRVAELAERIKKKLLKE---VWGEGEMTLAEAVKRRME--REGATLSTMESLTG--------GLLGAEITRVPGASR--------FYLGGVVSYSVGAKARFGVPQDLLSRTVSAETARAMAEAARSLFGST-----YALATTGVAGPDPLEGEPP--GTVYVALAGPT-----GAEVRRYRFPGDRETVRLRSVYAALALLVT----

General information: TITO was launched using: RESULT: Template: 4UUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1964 93935 47.83 245.26 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 47.83 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.247

(partial model without unconserved sides chains): PDB file : Tito_4UUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UUW-query.scw PDB file : Tito_Scwrl_4UUW.pdb: