Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------------------------MKTASKFSVMFFMLLALCGCWDVKDIEQLSFARGLAIDETNDHQYKLTYQNL--------LPQSEDSQASGKPEFVNVTSHG-KTILEAVSDVSIKDPPVY----------SDHLKVILLGEKLMRNQ--NVDQVLN------------HFIRDDELRRSSYLMAARGNAADVFTKGNPNQQQPMPSEKLIDLTTHSGYNGKIMIPLRIGRASVYSQNGYSYLIQAVKNEKGKAKYDGAGII--KRGSNKLVGFLSADETQTLSWVMGTIQGGVMPTTDKGHPITFEIKKSKTKIKPVI----ENGKPVFHISVKTKGILTEDQNPNENSFSKSYLHRLENIFEKKLERDVKQVMDKLQHEYKTDPVFLSDHIRIQHPDYWNKVKGHWDEIFSETDFKYDISFKIINFGTVGK---------------- 4ZLA Chain:A ((7-502)) MLKIKLEKTTFENAKAECSLVFIINKDFSHAWVKNKELLETFKYEGEGVFLDQENKILYAGVKEDDVHLLRESACLAVRTLKKLAFKSVKVGVYTCGAHNALLENLKALFLGLKLGLYEYDTFKSNKKESVLKEAIVALELHKSLEKSAKEALKYAEIMTESLNIVKDLVNTPPMIGTPVYMAEVAQKVAKENHLEIHVHDEKFLEEKKMNAFLAVNKASLSVNPPRLIHLVYKPKKAKKKIALVGKGLTYDC---GGLSLK---PADYMVTMKADKGG-GSAVIGLLNALAKLGVEAEVHGIIGATENMIGPAAYKPDDILISKEGKSIEVRNTDAEGRLVLADCLSYAQDLNPDVIVDFATLTGACVVGLGEFTSAIMGHNEELKNLFETSGLESGELLAKLPFN---------RHLKKLIESKIA-DVCNISSSRYGGAITAGLFLNEFIR-------DEFKDKWLHIDIAGPAYVEKEWDVNSFGASGAGVRACTAFVEELLKKA

General information: TITO was launched using: RESULT: Template: 4ZLA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1937 79115 40.84 226.04 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 40.84 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.094

(partial model without unconserved sides chains): PDB file : Tito_4ZLA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZLA-query.scw PDB file : Tito_Scwrl_4ZLA.pdb: