Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFVSMKELLEDAKREQYAIGQFNINGLQWTKAILQAAQKEQSPVIAAASDRLVDYLGGFKTIAAM-VGALIEDMAIT--VPVVLHLDHGSSAERCRQAIDAGFSSVMIDGSHQPIDENIAMTKEVTDYAAKHGVSVEAEVGTVGGMEDGLVGGVRYADIT---ECERIVKETNIDALAAALGSVHGKYQGEPNLGFKEMEAISRMTDIPLVLHGASGIPQDQIKKAITLGHAKININTECMVAWTDETRRMFQENSDLYEPRGYLTPGIEAVEETVRSKMREFGSAGKAAKQQVG 1GVF Chain:B ((2-283)) -SIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSPVILAGTP------GTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAMIDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVE--------SAFLTDPQEAKRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFVRRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKINVCGSANR-------

General information: TITO was launched using: RESULT: Template: 1GVF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1471 2394 1.63 8.93 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : 1.63 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_1GVF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GVF-query.scw PDB file : Tito_Scwrl_1GVF.pdb: