Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGTEKVTALKAKHQSKSSTGLSWFERIGYGFGDMSYNIIFQFVNAYLLFYYTDVGGIQPAVIATLFLVVRVLDAIFDPIMGLILDKTNTRWGKARPYLLWVAFPFALFTFLCFTTPH-F---------G--ETGNMVYAYVTYILLGMSFSMQTIPVNSLTGRMTNSVEERTVLTTTRMILVYIGILLSISCATPLATAIGG-EDQAFGFQMTALIYAAVSIVLNLFSFFTVRERIQPKK--RKKQ----------------G----IKKTLSV-L-FKNKPLLMLISSFLAF---------AIGFNIKLSTMVYYFTYNVNHKE-FVFMGTVLFFGAALISNLFIPFFSEKWGRKQVMIITAALSLIS-YAGLHF-TPYSSIPLIFIWLF-ASGFFTT-PLNTLAWGMVADCVDYAEWKTGIRADGVVISSMSFINKLGVALAGSFSAIYLGIAGYVANTDQTVASLNAIKNMNALIPGFFILLSIILIAFYPLTEKRYKHIISELEQRSAR 4GC0 Chain:A ((51-431)) --------------------------------------------------------SAANSLLGFCVASALIGCIIGGALGGYCSNR----FGRRDSLK-IAAVLFFISGVGSAWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPA-HIRGKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLF-LMLLYTVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHS-LDHGRKTGGRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFK-TLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGI-VALLSMLFYVAAFAMSWGPVCWVLLSEIFPN-------AIRGKALAIAVAAQWLANYFVSWTFPMMDK------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4GC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1391 -181085 -130.18 -548.74 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -130.18 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_4GC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GC0-query.scw PDB file : Tito_Scwrl_4GC0.pdb: