Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSLKGKRIGFGLTGSHCTYEAVFPQIEELVNEGAEVRPVVTFNVKS--TNTRFGEGAEWVKKIEDLTGYEAIDSIVKAEPLGPKLPLDCMVIAPLTGNSMSKLANAMTDSPVLMAAKATIRNNRPVVLGISTNDALG--LNGTNLMRLMSTKNIFFIPF--GQDDPFKKPNSMVAKMDLLPQTIEKALMHQQLQPILVENYQGND 1E20 Chain:A ((18-202)) ----RKPRVLLAASGSVAAIKFG--NLCHCFTEWAEVRAVVTKSSLHFLDKLSLPQEVTLYTDEDEWSSWNKIGDPVLHIELRRWA--DVLVIAPLSANTLGKIAGGLCDN-LLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLACGDYGNGAMAEPSLIYSTVRLFWESQAH------------

General information: TITO was launched using: RESULT: Template: 1E20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 926 -125887 -135.95 -703.28 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -135.95 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_1E20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E20-query.scw PDB file : Tito_Scwrl_1E20.pdb: