Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIIRKISHFAGQTFGIWVIVFAVLGFSFP-------SLFTWISSYITIFLGIIMFGMGLTLQAD----DFKELVRKPWQVIIGVIAQY-TIMPLVAFGLAFGLHLPAEIAVGVILVGCCPGGTASNVMTFLAKGNTALSVAVTTISTLLAPVVTPLL-IMLFAKEWLPVSPGSLFISILQAVLFPIIAGLIVKMFFRKQVAKAVHALPLVSVIGIVAIVSAVVSGNRENLLQSGLLIF-SVVILHNGIGYLLGFLCAKLLKMDYPSQKAIAIEVGMQNSGLGAALATAHFSPLSAVPSAIFSVWHNLSGSMLATYWSKKVKKKQAGSKSSNLSL------ 3PT5 Chain:A ((4-326)) --IISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQLARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARKLLPFIPDN--AGILIVPCCRGGSA---FTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRTRAALVKNPQNKFLG-------------VCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNDAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGARGLTNAPDEDPDDLSTGYYGSAYRSP-ENWTTALRSSHFSSAARRGIISDRFVEAILQFWRER

General information: TITO was launched using: RESULT: Template: 3PT5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1782 -88104 -49.44 -293.68 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -49.44 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_3PT5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PT5-query.scw PDB file : Tito_Scwrl_3PT5.pdb: