Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MSLLIDIVDETGSV-SEEMLKEVENLLQFAAEREGVQDQAEVSVTIVSNDDIHQINKEYRGKDAPTDVISFALEEEGEGEI------EIVGAEMPPVLGDIIISADRTREQAEEYNHSFKRELGFLAVHGFLHLLGYDHMTKEEEEEMFTK---------------QKELLDAYGLKRS 456C Chain:A ((7-166)) TLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDV-----------TPLNFTRLHD--------GIADIMISFGIKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSS---KGYNLFLVAAHEFGHSLGLDH-SKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPG--

General information: TITO was launched using: RESULT: Template: 456C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 610 44475 72.91 336.93 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 72.91 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_456C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-456C-query.scw PDB file : Tito_Scwrl_456C.pdb: