Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIIAGTVVKGKQLG-RKLGFPTANVDAKIH--GLRNGVYGVLATVN------------HQFHLGVMNIGVKPTVGSNLEKTLEIFLFDFHRDIYGEKIECSILFKIREERRFDSLESLTKQIKKDISCVAKRFELIGIMAPNKKESLLSHQELNLPDLCFYKKCNNLYGVNRGVYNVIDNWFFEYGITQVAYRRIYILSFLSFLKEDNPKVSSKYIRFGAGGLADKLNRFISSYVEESEENILG 5A89 Chain:A ((4-149)) HFYVTGPVVRGAGRGGKELGFPTANQYFHDTVALPADGVYAGWLTILPTEAPVSGNMEPEVAYAAAISVGTNPTFGDE-QRSVESFVLDRDADLYGHDVKVEFVDHVRAMEKFDSVEQLLEVMAKDVQKTRTLLAQDVQAHKMAPET--------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5A89.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 627 20735 33.07 158.28 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 33.07 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.548

(partial model without unconserved sides chains): PDB file : Tito_5A89.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5A89-query.scw PDB file : Tito_Scwrl_5A89.pdb: