Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence M-PDSGLLYKEWRQNKVALVITILVFI-LGNPLSILNMYLIYQGCVTGKENWVGPCVFSV--DYLNSSFISLFWIWGVVLAVSQLGIERSKSFFDFTLSLPYTRGQIF-HAKFLTGGMVIVVPQLIGYVLSVLLIMLLKPDQAVYFHNYSLGMIIVS--MLAYSLVMAGGALTGNSFAQLLVSFTVAISPFLLISLPVINLEILFGGSIDFIHGPVPKWVQYFIPIIYVDSKWAENSPYYLVIPAIMTIIFYIIGYISFVKMSNERNGYFFLWKPLNRPVQIIVIIIGIMGFGYFGFTASESFAGYLIGMGTGAVIGFLISYFAIYKKMKLL--------------------------------------------------- 4P5F Chain:A ((8-374)) LTPTEAAVLRELRLHRPQLPLDTLLFTDPNKDPDDVVTYTIAKQLQADGFLRLTDVVVTLGDADMRSQRAQLAKGVFDRLALPDVRVARGQDY-------PMTSTQAREHSKFLAEGAALRAA-----PDAVHTDGVRAMCERLATSPHKLGMVVIAGMTDASALLAEAGDLVREKVASITIMGGIDPARDADGLVQPDTRAYNNATDIHAARALYRRAQQLGIPLRILTKEAAYKAA---VPPAFYEGIARNGHPVGEYLRDVQKNALKGLWEGIQANL-------------IPGLDTAWFFRTFVAGALSFDAIWPQVTKLNLYDPLTLLAALPGTARLLFQPTPMHREGASPVEHVGHAEVVRPEKARLLLSALAKAALV

General information: TITO was launched using: RESULT: Template: 4P5F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1490 -104747 -70.30 -352.68 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -70.30 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.164

(partial model without unconserved sides chains): PDB file : Tito_4P5F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P5F-query.scw PDB file : Tito_Scwrl_4P5F.pdb: