Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATIKDVAGAAGVSVATVSRNLNDNGYVHEETRTRVIAAMAKLNYYPNEVARSLYKRESRLIGLLLPDITNPFFPQLARGAEDELNREGYRLIFGNSDEELKKELEYLQTFKQNHVAGII---AATNYPDLEEYSGMNYPVVFLDRTLEGA---PSVSSDGYTGVKLAAQAIIHGKSQRITLLRGPAHLPTAQ-DRFNGALEILKQAEV---DFQVIETASFSIKDAQSMAKELFASYPATDGVIASNDIQAAAVLHEALRRGKNVPEDIQIIGYDDIPQSGLLFPPLSTIKQPAYDMGKEAAKLLLGIIKKQPLAETAIQMPVTYIGRKTTRKED 1ZVV Chain:B ((3-332)) -VTIYDVAREASVSMATVSRVVNGNPNVKPSTRKKVLETIERLGYRPNAVARGLASKKTTTVGVIIPDISNIFYAELARGIEDIASMYKYNIILSNSDQNQDKQLHLLNNMLGKQVDGIIFMSGNVTEEHVEELKKSPVPVV-LAASIESTNQIPSVTIDYEQAAFDAVQSLIDSGHKNIAFVSGTLEEPINHAKKVKGYKRALTESGLPVRDSYIVE-GDYTYDSGIEAVEKLLEEDEKPTAIFVGTDEMALGVIHGAQDRGLNVPNDLEIIGFDNTRLSTMVRPQLTSVVQPMYDIGAVAMRLLTKYMNKETVDSSIVELPHRIEFRQSTK---

General information: TITO was launched using: RESULT: Template: 1ZVV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1655 -5970 -3.61 -18.65 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -3.61 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_1ZVV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZVV-query.scw PDB file : Tito_Scwrl_1ZVV.pdb: