Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MNHLPEKMRPYRDLLEKSAKEYVKLNVRKGKTGRYDSKIAGDPYFPKHETYPTDENGQPMKLLAQINFSHIPQLDGYPSSGILQFYISVHDDVYGLNFDDRCEQKNFRVIYFENIVENDDELVSDFSFIGTGECDFPILSEAAVEPVKSSEWVLPTDFQFEQYTGMETMEFFGQFGEDEEDIYNELAENGFGHKIGGYASFTQH--DPREYA------YKEHTIMLLQ---IDSDDDIDSMWGDVGIANFFITPEDLRKKDFSNVLYNWDCS----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 4Z24 Chain:A ((4-652)) NLTGDIVIIGAGAAGSLLAHYLARFSNMKIILLEAGHSHFNDPVVTDPMGFFGKYNPPNENISMSQNPSYSWQGAQEPNTGAYGNRPIIAHGMGFGGSTMINRLNLVVGGRTVFDNDWPVGWKYDDVKNYFRRVLVDINPVRDNTKASITSVALDALRIIAEQQIASGEPVDFLLNKATGNVPNVEKTTPDAVPLNLNDYEGVNSVVAFSSFYMGVNQLSDGNYIRKYAGNTYLNR---NYVDENGRGIGKFSGLRVV---------SDAVVDRIIFKGNRAVGVNYIDR-EGIMHYVKVNKEVVVTSGAFYTPTILQRSGIGDFTYLSSIGVKNLVYNNPLVGTGLKN-HYSPVTITRVHGE-PSEVSRFLSNMAANPTNMGFKGLAELGFHRLDPNKPANANTVTYRKYQLMMTAGVGIPAEQQYLSGLSPSSNNLFTLIADDIRFAPEGYIKIGTPNIPRDVPKIFFNTFVTYTPTSAPADQQWPIAQKTLAPLISALLGYDIIYQTLMSMNQTARDSGFQVSLEMVYPLNDLIYKLHNGLATYGANWWHYFVPTLVGDDTPAGREFADTLSKLSYYPRVGAHLDSHQGCSCSIGRTVDSNLKVIGTQNVRVADLSAAAFPPGGNTWATASMIGARAVDLILGFPYLRDLPVNDVPILNVN

General information: TITO was launched using: RESULT: Template: 4Z24.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1018 24547 24.11 99.78 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 24.11 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.141

(partial model without unconserved sides chains): PDB file : Tito_4Z24.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z24-query.scw PDB file : Tito_Scwrl_4Z24.pdb: