Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKNILLVGMLVLLLMFVSACSGTASKGSSSDSASEKTEMRTYKSPKGNVNIPAHPKRIVTDF--YAGELLSVGANVVGSGSWS--FDN----PFL--KSKLK---NVKDVGD-PISVEKVMELQPDLIVVMNE------ENVDKLKKIAPTVVIPYNTAKNVEDTVSMFGDIAGAKDQAKSFMADFNKKAEAAKKKIAGVIDKDATFGIYENTDKGEFWVFNDNGGRGGQAVYNALGLKAPEKIEQDV-----IKKGEMKQLSQEVIPE-YAADYMFITDYNPKGESKTLDKLENSSIWKNLDAVKHNRVFINDFDSFYPYDPISVSKQVDIITDMLIKRAEENKK 3TLK Chain:A ((27-320)) ------------------------------------ADWPRQITDSRGTHTLESQPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLYIGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYD-DKSWQSLLTQLGEITGHEKQAAERIAQFDKQLAAAKEQIKLP-PQPVTAIVYTAAA-HSANLWTPE-SAQGQ-MLEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQ----KDADAIYANPLLAHLPAVQNKQVYALGTETF-RLDYYSAMQVLDRLKALFLE-------

General information: TITO was launched using: RESULT: Template: 3TLK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1372 73117 53.29 272.82 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 53.29 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_3TLK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TLK-query.scw PDB file : Tito_Scwrl_3TLK.pdb: