Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLPAKTFVILCILFLLDLCFSYIRHEWHSQNALQDMPVPSDLHPIVKQNADALKAAAANKGIDVVITEGFRSFKEQDELYKQGRTKKG------------NIVTYARGGESYHNYGLAIDFALQKKDGSIIWDMEYDGNQNGKSDWLEVVEIAKTLGF--EWGGDWK----RFKDYPHLEMIPN 5HNM Chain:A ((17-175)) ----------EWSLILVNRQNPIPA---QYDVELEQLSNGERIDIRISPYLQDLFDAARADGVYPIVASGYRTTEKQQEIMDEKVAEYKAKGYTSAQAKAAAETWVAVPGTSEHQLGLAVDINADGI----HST------GNEVYRW--LDENSYRFGFIRRYPPDKTEITGVSNEPWHYRYVG-

General information: TITO was launched using: RESULT: Template: 5HNM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 584 -47795 -81.84 -338.97 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -81.84 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.274

(partial model without unconserved sides chains): PDB file : Tito_5HNM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HNM-query.scw PDB file : Tito_Scwrl_5HNM.pdb: