Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHLSILDQAPVSKGESPVTTLQHSVELAQLSEQWGYKRYWFAEHHSTKGLASTAPEIMIARIAAQTNTIRVGSGGVLLPQYSPFKVAETFRQLEALYPNRIDLGVGRSPGGTTKTRLALTDGVKKSLTEFNRQLQDVSYFLTDSLP-PDHPY---AGIKAAPLI--GTAPELWVLGLGENSARRAAHQGIGYVFGHFINPER-GENAFRIYRESFRPSAHF-SNPSALFTIFVICAKTDEEAEELALSQDLWLLRV--GK-GLDSRVPSIEEAKAHPYTASDKKLIEENRKRMVIGSPTTVKQQLLDLTGCYETNEIMVLCNV-FDFEAKKESYERLAELFL 1LUC Chain:A ((1-348)) -MKFGNFLLTYQPPELSQTEVMKRLVNLGKASEGCGFDTVWLLEHHFTEFGLLGNPYVAAAHLLGATETLNVGTAAIVLPTAHPVRQAEDVNLLDQMSKGRFRFGICRGLYD--KDFRVFGTDMDNSRALMDCWYDLMKEGFNEGYIAADNEHIKFPKIQLNPSAYTQGGAPVYVVAESASTTEWAAERGLPMILSWIINTHEKK-AQLDLYNEVATEHGYDVTKIDHCLSYITSVDHDSNRAKDICRNFLGHWYDSYVNATKIF--------------------RIDYSYEINPVGTPEECIAIIQQDIDATGIDNICCGFEANGSEEEIIASMKLFQSDVM

General information: TITO was launched using: RESULT: Template: 1LUC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1624 -169067 -104.10 -550.71 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -104.10 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_1LUC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LUC-query.scw PDB file : Tito_Scwrl_1LUC.pdb: