Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQVEENEHIQTLVYQLNKEGKSICGDSFFMKADDKELICAVADGLGSGSLANESSAAIKDLVENYAS-----------EDVESIIERCNQAMKN---------KRGATASILKINFEQRQFTYCSVGNVRFILHSPSGESFYPLPISGY----------LSGKP--QKYKTHTATYEKGSKFIIHTDGLNVPDIRSHLK---KGQSVEEISNSLKMYT--TSRKDDLTYILGQLS 2J82 Chain:A ((13-240)) ---------------------RKSNQDAFYID-EKHQRFFIVADGM---AGGEEASRLAVDHIRQYLETHLEDLQHDPVTLLRQAF-AANHAIVEQQRQNSARADMGTTAVVILLDEKGDRAWCAHVGDSRIYRWRKD-QLQQITSDHTWIAQARHVLSQCLGREDLSQIDIQPIDLEPGDRLLLCSDGLTEELTDDVISIYLSEPNVQKAAAALVDAAKTHGGRDNVTVVVISV-

General information: TITO was launched using: RESULT: Template: 2J82.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 977 -115710 -118.43 -676.66 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -118.43 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_2J82.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J82-query.scw PDB file : Tito_Scwrl_2J82.pdb: