Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAGKSYRKKMKQRRMNMKISKYALGILMLSLVFVLSACGNNNSTKESTHDNHSDSSTHEEMDHSGSADVPEGLQESKNPKYKVGSQVIINTSHMKGMKGA--EATVTGAYDTTAYVVSYTPTNGGQRVDHHKWVIQEEIKDAG--DKTLQPGDQVILEASHMKG--MKGATAEIDSAEKTTVYMVDYTSTTSGEKVKNHKWVTEDELSAK 3H8Z Chain:A ((10-124)) --------------------------------------------------------------------------------AYFQGLPVEVR-----GSNGAFYKGFVKDVHE-DSVTIFFEN--NW---QSERQIPFGDVRLPPPA--EITEGDEVEVYSRA---EPCGWWLARVRMMKG-DFYVIEYAAC--------NEIVTLERLR--

General information: TITO was launched using: RESULT: Template: 3H8Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 389 -38049 -97.81 -388.26 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -97.81 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_3H8Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3H8Z-query.scw PDB file : Tito_Scwrl_3H8Z.pdb: