Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLMNEFEKACETLRKFMAYMLEK-DMKSWTELWDENAVFEFPYAPEGSPKRIEGKAAIYDYIKDYPKQIHLSSFTAPTVYRSADSNTVIAEFQCDGHVIETGLPYRQSYISVIETRDGRIVRYRDYWNPLVVKEAFGGSFLQTEESGK 4J9A Chain:E ((2-124)) ---NAKEIVVHALR-----LLENGDARGWSDLFHPEGVLEYPYPPPGYKTRFEGRETIWAHMRLFPEYMTIR-FTDVQFY-----DLAIGEFHGDGVL----GKLAYDYIAVWRT---QILLYRLFFNPLRV----------------

General information: TITO was launched using: RESULT: Template: 4J9A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 479 -62589 -130.67 -568.99 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain E : 0.74 3D Compatibility (PKB) : -130.67 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_4J9A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J9A-query.scw PDB file : Tito_Scwrl_4J9A.pdb: