Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MAVNFYDVAYDLENALRGSE-EFTRLKNLYDEVNADESAKRMFENFR---DVQLRLQQKQMAGEEITQEEVTQAQKTVALVQQHEKI---SQLMEAEQRMSMLIGELNKIIMKPLEEL--YGSVEG---------------------------------------------------------- 5DFM Chain:A ((1-527)) KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALAAAQTNAAAMQQPKRNFDLYKLITDKQIDFQVADLIQDE------QSSFVSVRIYGQFKCFVPKSTIQEQLDKIKNLKELAKNKIFKFLSEYNYYGYFKVQQHQFILNLENAQREASLAVDDFYFINGRIYKTNHDILILQAHHVYQMQKPTLQLLQA

General information: TITO was launched using: RESULT: Template: 5DFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 274 -4909 -17.91 -44.22 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -17.91 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_5DFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DFM-query.scw PDB file : Tito_Scwrl_5DFM.pdb: