Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKE-----TAWMKRKQLLYTEERKWEYGTILIEDGICLI-----------------------------------ENGEGD----ILLADSLQHSPIWIHHKGKWEQAGFQDKLVLACGAENISLSGGERIRY-EKSVKRPLMALLDSLDDETFLAFLQ----HLHSFGLS----VFD----CVFSYNKGVFSNTSAGQGVSFYHFSN----DTAQCAMQHHYNYSSEGTGDRFEWTASNGKRSIMYTAVQRGRK- 1MRO Chain:C ((1-247)) ---AQYYPGTTKVAQNRRNFCNPEYELEKLREISDEDVVKILGHRAPGEEYPSVHPPLEEMDEPEDAIREMVEPIDGAKAGDRVRYIQFTDSMYFAPAQPYVRSRAYLCRYR-------GADAGTLSGRQIIETRERDLEKISKELLETEFFDPARSGVRGKSVHGHSLRLDEDGMMFDMLRRQIYNKDTGRVEMVKNQIGDELDEPVDLGEPLDEETLMEKTTIYRVDGEAYRDDVEAVEIMQRIHVLRSQGGFNL

General information: TITO was launched using: RESULT: Template: 1MRO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 776 -22034 -28.39 -119.10 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain C : 0.63 3D Compatibility (PKB) : -28.39 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.180

(partial model without unconserved sides chains): PDB file : Tito_1MRO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MRO-query.scw PDB file : Tito_Scwrl_1MRO.pdb: