Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------MEIERINEHTVKFYMSYGDIEDRGFDREEIWYNRERSEELFWEVMDE-----VHEEEEFAVEGPL-------WIQVQALDKG---------LEIIVTKAQLSK------DGQKLELPIPEDKKQEPASEDLDALLDDFQKE--EQAVNQEEKEQKLQF-VLRFGDFEDVI-----------------------SLSKLNVNGSKTTLYSFENRYYLYVDFCN------MTDEEVENQ-----------LSILLEYATE----SSISIHRLEE--------YGKLIISEHALETIKKHFAS-- 4P5W Chain:A ((31-394)) TKRADPAELRTIFLKYASIEKNG-EFFMSPNDFVTRYLNIFSQ--PNPKTVELLSGVVDQTKDGLISFQEFVAFESVLCAPDALFMVAFQLFDKAGKGEVTFEDVKQVFGQTTIHQHIPFNWDSEFVQLHFGKERKRHLTYAEFTQFLLEIQLEHAKQAFVQRDNARTGRVTAIDFRDIMVTIRPHVLTPFVEECLVAAAGGTTSHQVSFSYFNGFNSLLNNMELIRKIYSTLAGTRKDVEVTKEEFVLAAQKFGQVTPMEVDILFQLADLYEPRGRMTLADIERIAPPNPDHVGGYKLAVATFAGIENKFGLYL

General information: TITO was launched using: RESULT: Template: 4P5W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 706 -87018 -123.25 -404.73 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -123.25 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_4P5W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P5W-query.scw PDB file : Tito_Scwrl_4P5W.pdb: