Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEVKRRNI-------QDPKEVQSVISEKLVEIYNSGD--EQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFRAGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPHEVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLFSDLVEKADD 3E70 Chain:C ((56-324)) ---------------------------------------------------LDELEIDLLEADVA----LEVVDALREKIKQKLVGKKVRIG--GKIIEEAVKEAVSEILETSRRIDLIEEIRKAE-KPYVIMFVGFNGSGKTTTIAKLANWLKNHGFSVVIAASDTFRAGAIEQLEEHAKRIGVKVIKHSYGADPAAVAYDAIQHAKARGIDVVLIDTAGRSETNRNLMDEMKKIARVTK------PNLVIFVGDALAGNAIVEQARQFNEAVKIDGIILTKLDADARGGAALSISYVIDAPILFVGVGQGYDDLRPFEKEWFLERIFG--------

General information: TITO was launched using: RESULT: Template: 3E70.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1358 -164108 -120.84 -638.55 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -120.84 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_3E70.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E70-query.scw PDB file : Tito_Scwrl_3E70.pdb: