Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAQNNENALPDITKSITLEAPIQKVWETVSTSEGIAKWFMPN-DFQLKEGQEFHLQSPFGPSPCKVLAVQAPTELSFEWDTE------GWVVTFQLEDLGEKTGFTLIHSGWKEPNEVIGKANEKSSVVRGKMDGGWTGIVNERLRKAVEE 2M89 Chain:A ((2-131)) ---------VNINHRIGIKASPEKIYQALTTDDGLKKWWTNDISGAGVVGSTIKFRFNGGGPDFKVTKLIPNKTVCWQHAGNMPESWMGTEISFQLETVENQTFVRFTHSNWHETTD-----------FMAHCNTKWAV-FLLSLKDALEI

General information: TITO was launched using: RESULT: Template: 2M89.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 481 17157 35.67 139.48 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 35.67 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_2M89.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2M89-query.scw PDB file : Tito_Scwrl_2M89.pdb: