Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVIDDFEKLDIRTGTIVKAEEFPEARVPAIKLVIDFGTEIGIKQSSAQITKRYKPEGLINKQVIAVVNFPPRRIAGFKSEVLVLGGIPGQGDVVLLQPDQPVPNGTKIG 2CWP Chain:A ((6-112)) LYDVDEFWKFQMKVGLVKKAEKIKRTKK-LIKLIVDFGNE--ERTIVTGIADQIPPEELEGKKFIFVVNLKPKKFSGVESQGMLILAETEDGKVYLIPVPEEVPVGARVW

General information: TITO was launched using: RESULT: Template: 2CWP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 466 -53019 -113.77 -495.50 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -113.77 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_2CWP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CWP-query.scw PDB file : Tito_Scwrl_2CWP.pdb: