Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFEKMKQWMEFAQQMYGGDFWKQVFDEDQKTPFMTNGQSPFPFAQQDQRGKGDASFPSMDIVDTVAEVQFLIYLPGYRKQDVHILSYG-DYLVVKGQRFSYFN--EQDFRQKEGKYGSFEKKIPLSDHLHG-KMNAIFKDGILYITIQKDEGQA---KTIVIDD 1GME Chain:A ((40-151)) -----------------------------------------------------AFANARMDWKETPEAHVFKADLPGVKKEEVKVEVEDGNVLVVSGERTKEKEDKNDKWHRVERSSGKFVRRFRLLEDAKVEEVKAGLENGVLTVTVPKAEVKKPEVKAIQISG

General information: TITO was launched using: RESULT: Template: 1GME.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 369 4633 12.56 44.12 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 12.56 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_1GME.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GME-query.scw PDB file : Tito_Scwrl_1GME.pdb: