Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MIQGFYKDQKLH-----LLEDPMQQYTVMKVEENAVCVYRWIDDYRHKIERFTDVEEAKKLLGEGWPKQ------------------------- 1I35 Chain:A ((1-95)) CILRFIACNGQTRAVQSRGDYQKTLAIALKKFSLEDASKFIVCVSQ-----SSRIKLITEEEFKQICFNSSSPERDRLIIVPKEKPCPSFEDLRRSWEIE

General information: TITO was launched using: RESULT: Template: 1I35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 198 10892 55.01 184.60 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 55.01 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.305

(partial model without unconserved sides chains): PDB file : Tito_1I35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1I35-query.scw PDB file : Tito_Scwrl_1I35.pdb: